Review



intravenous treatment with srage  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    R&D Systems intravenous treatment with srage
    RAGE inhibition restores alveolar fluid clearance after acid-induced lung injury. Measurement of net alveolar fluid clearance (AFC) rate as a marker of epithelial function in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant <t>sRAGE</t> (HCl + sRAGE) (n = 12 per group at each time point). Values are reported as box and whisker plots. The Kruskal-Wallis test, with post-hoc Bonferroni test for pairwise comparisons were used. P = 0.02 for the overall multiple group test; no statistical difference was found in post-hoc tests.
    Intravenous Treatment With Srage, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/intravenous+treatment+with+srage/Recombinant+Human+RAGE+Fc+Chimera+Protein%2C+CF/pmc06592897-117-15-28
    Average 94 stars, based on 20 article reviews
    intravenous treatment with srage - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets"

    Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-45798-5

    RAGE inhibition restores alveolar fluid clearance after acid-induced lung injury. Measurement of net alveolar fluid clearance (AFC) rate as a marker of epithelial function in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group at each time point). Values are reported as box and whisker plots. The Kruskal-Wallis test, with post-hoc Bonferroni test for pairwise comparisons were used. P = 0.02 for the overall multiple group test; no statistical difference was found in post-hoc tests.
    Figure Legend Snippet: RAGE inhibition restores alveolar fluid clearance after acid-induced lung injury. Measurement of net alveolar fluid clearance (AFC) rate as a marker of epithelial function in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group at each time point). Values are reported as box and whisker plots. The Kruskal-Wallis test, with post-hoc Bonferroni test for pairwise comparisons were used. P = 0.02 for the overall multiple group test; no statistical difference was found in post-hoc tests.

    Techniques Used: Inhibition, Marker, Recombinant, Whisker Assay

    RAGE inhibition improves arterial oxygenation after acid-induced lung injury. Arterial oxygen tension (PaO 2 )/inspiratory oxygen fraction (FiO 2 ) in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group at each time point). Individual values are reported. Time × group interaction and post-hoc comparisons were verified using random effects models to analyse longitudinal evolution of variables.
    Figure Legend Snippet: RAGE inhibition improves arterial oxygenation after acid-induced lung injury. Arterial oxygen tension (PaO 2 )/inspiratory oxygen fraction (FiO 2 ) in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group at each time point). Individual values are reported. Time × group interaction and post-hoc comparisons were verified using random effects models to analyse longitudinal evolution of variables.

    Techniques Used: Inhibition, Recombinant

    RAGE inhibition decreases alveolar-capillary permeability after acid-induced lung injury. Values are reported as box and whisker plots. ( A ) Level of total protein in the bronchoalveolar lavage (BAL) fluid from uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group). The Kruskal-Wallis test, with post-hoc Bonferroni test for pairwise comparisons were used. ( B ) Extravascular lung water, as measured by transpulmonary thermodilution (Picco + , Pulsion SA) and indexed to body weight, in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group at each time point). Time × group interaction and post-hoc comparisons were verified using random effects models to analyse longitudinal evolution of variables.
    Figure Legend Snippet: RAGE inhibition decreases alveolar-capillary permeability after acid-induced lung injury. Values are reported as box and whisker plots. ( A ) Level of total protein in the bronchoalveolar lavage (BAL) fluid from uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group). The Kruskal-Wallis test, with post-hoc Bonferroni test for pairwise comparisons were used. ( B ) Extravascular lung water, as measured by transpulmonary thermodilution (Picco + , Pulsion SA) and indexed to body weight, in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group at each time point). Time × group interaction and post-hoc comparisons were verified using random effects models to analyse longitudinal evolution of variables.

    Techniques Used: Inhibition, Permeability, Whisker Assay, Recombinant

    RAGE inhibition decreases alveolar inflammation after acid-induced lung injury. Measurement of bronchoalveolar lavage (BAL) levels of ( A ) tumour necrosis factor (TNF)-α, ( B ) interleukin (IL)-6, ( C ) IL-1β and ( D ) IL-18 in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group). Values are reported as box and whisker plots. The Kruskal-Wallis test, with post-hoc Bonferroni test for pairwise comparisons were used.
    Figure Legend Snippet: RAGE inhibition decreases alveolar inflammation after acid-induced lung injury. Measurement of bronchoalveolar lavage (BAL) levels of ( A ) tumour necrosis factor (TNF)-α, ( B ) interleukin (IL)-6, ( C ) IL-1β and ( D ) IL-18 in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group). Values are reported as box and whisker plots. The Kruskal-Wallis test, with post-hoc Bonferroni test for pairwise comparisons were used.

    Techniques Used: Inhibition, Recombinant, Whisker Assay

    (A ) RAGE inhibition decreases histological features of lung injury. Lung injury scores were higher in acid-injured (HCl) than in uninjured piglets (Sham) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group). Values are reported as box and whisker plots. The Kruskal-Wallis test, with post-hoc Bonferroni test for pairwise comparisons were used. (B – E) Representative hematoxylin and eosin–stained sections at x20 original magnification of (B) uninjured piglets (Sham), (C) acid-injured piglets (HCl), acid-injured piglets treated with (D) RAGE antagonist peptide (HCl + RAP) or (E) recombinant sRAGE (HCl + sRAGE). There was greater cellularity consisting mainly of neutrophils (black arrowheads), more alveolar wall thickening (white arrowheads), and more areas of atelectasis and increased alveolar disruption, proteinous debris, and hemorrhage (black arrows) in untreated, acid-injured than in uninjured or treated piglets. Scale bars, 100 µm.
    Figure Legend Snippet: (A ) RAGE inhibition decreases histological features of lung injury. Lung injury scores were higher in acid-injured (HCl) than in uninjured piglets (Sham) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group). Values are reported as box and whisker plots. The Kruskal-Wallis test, with post-hoc Bonferroni test for pairwise comparisons were used. (B – E) Representative hematoxylin and eosin–stained sections at x20 original magnification of (B) uninjured piglets (Sham), (C) acid-injured piglets (HCl), acid-injured piglets treated with (D) RAGE antagonist peptide (HCl + RAP) or (E) recombinant sRAGE (HCl + sRAGE). There was greater cellularity consisting mainly of neutrophils (black arrowheads), more alveolar wall thickening (white arrowheads), and more areas of atelectasis and increased alveolar disruption, proteinous debris, and hemorrhage (black arrows) in untreated, acid-injured than in uninjured or treated piglets. Scale bars, 100 µm.

    Techniques Used: Inhibition, Recombinant, Whisker Assay, Staining, Disruption

    Related Articles

    Recombinant:

    Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets
    Article Snippet: Animals with HCl-induced lung injury and receiving intravenous treatment with RAP (EMD Millipore, Burlington, MA, USA) (3 μg.kg −1 ) defined the “RAP group” (n = 12). .. The “sRAGE group” (n = 12) included animals with HCl-induced lung injury that also received intravenous treatment with sRAGE (3 mg.kg −1 ) (Recombinant Human RAGE Fc Chimera, R&D Systems, Minneapolis, MN). ..



    Similar Products

    intravenous treatment with srage  (R&D Systems)
    94
    R&D Systems intravenous treatment with srage
    RAGE inhibition restores alveolar fluid clearance after acid-induced lung injury. Measurement of net alveolar fluid clearance (AFC) rate as a marker of epithelial function in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant <t>sRAGE</t> (HCl + sRAGE) (n = 12 per group at each time point). Values are reported as box and whisker plots. The Kruskal-Wallis test, with post-hoc Bonferroni test for pairwise comparisons were used. P = 0.02 for the overall multiple group test; no statistical difference was found in post-hoc tests.
    Intravenous Treatment With Srage, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/intravenous+treatment+with+srage/Recombinant+Human+RAGE+Fc+Chimera+Protein%2C+CF/pmc06592897-117-15-28
    Average 94 stars, based on 1 article reviews
    intravenous treatment with srage - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    intravenous treatment with srage  (Santa Cruz Biotechnology)
    95
    Santa Cruz Biotechnology intravenous treatment with srage
    Measurement of net alveolar fluid clearance (AFC) rate as a marker of epithelial function in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl+RAP) or recombinant <t>sRAGE</t> (HCl+sRAGE) (n = 12 per group at each time point). Values are reported as medians and interquartile ranges.
    Intravenous Treatment With Srage, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/intravenous+treatment+with+srage/RAGE/bio_rxiv__405423-49-13-24
    Average 95 stars, based on 1 article reviews
    intravenous treatment with srage - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    RAGE inhibition restores alveolar fluid clearance after acid-induced lung injury. Measurement of net alveolar fluid clearance (AFC) rate as a marker of epithelial function in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group at each time point). Values are reported as box and whisker plots. The Kruskal-Wallis test, with post-hoc Bonferroni test for pairwise comparisons were used. P = 0.02 for the overall multiple group test; no statistical difference was found in post-hoc tests.

    Journal: Scientific Reports

    Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets

    doi: 10.1038/s41598-019-45798-5

    Figure Lengend Snippet: RAGE inhibition restores alveolar fluid clearance after acid-induced lung injury. Measurement of net alveolar fluid clearance (AFC) rate as a marker of epithelial function in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group at each time point). Values are reported as box and whisker plots. The Kruskal-Wallis test, with post-hoc Bonferroni test for pairwise comparisons were used. P = 0.02 for the overall multiple group test; no statistical difference was found in post-hoc tests.

    Article Snippet: The “sRAGE group” (n = 12) included animals with HCl-induced lung injury that also received intravenous treatment with sRAGE (3 mg.kg −1 ) (Recombinant Human RAGE Fc Chimera, R&D Systems, Minneapolis, MN).

    Techniques: Inhibition, Marker, Recombinant, Whisker Assay

    RAGE inhibition improves arterial oxygenation after acid-induced lung injury. Arterial oxygen tension (PaO 2 )/inspiratory oxygen fraction (FiO 2 ) in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group at each time point). Individual values are reported. Time × group interaction and post-hoc comparisons were verified using random effects models to analyse longitudinal evolution of variables.

    Journal: Scientific Reports

    Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets

    doi: 10.1038/s41598-019-45798-5

    Figure Lengend Snippet: RAGE inhibition improves arterial oxygenation after acid-induced lung injury. Arterial oxygen tension (PaO 2 )/inspiratory oxygen fraction (FiO 2 ) in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group at each time point). Individual values are reported. Time × group interaction and post-hoc comparisons were verified using random effects models to analyse longitudinal evolution of variables.

    Article Snippet: The “sRAGE group” (n = 12) included animals with HCl-induced lung injury that also received intravenous treatment with sRAGE (3 mg.kg −1 ) (Recombinant Human RAGE Fc Chimera, R&D Systems, Minneapolis, MN).

    Techniques: Inhibition, Recombinant

    RAGE inhibition decreases alveolar-capillary permeability after acid-induced lung injury. Values are reported as box and whisker plots. ( A ) Level of total protein in the bronchoalveolar lavage (BAL) fluid from uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group). The Kruskal-Wallis test, with post-hoc Bonferroni test for pairwise comparisons were used. ( B ) Extravascular lung water, as measured by transpulmonary thermodilution (Picco + , Pulsion SA) and indexed to body weight, in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group at each time point). Time × group interaction and post-hoc comparisons were verified using random effects models to analyse longitudinal evolution of variables.

    Journal: Scientific Reports

    Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets

    doi: 10.1038/s41598-019-45798-5

    Figure Lengend Snippet: RAGE inhibition decreases alveolar-capillary permeability after acid-induced lung injury. Values are reported as box and whisker plots. ( A ) Level of total protein in the bronchoalveolar lavage (BAL) fluid from uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group). The Kruskal-Wallis test, with post-hoc Bonferroni test for pairwise comparisons were used. ( B ) Extravascular lung water, as measured by transpulmonary thermodilution (Picco + , Pulsion SA) and indexed to body weight, in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group at each time point). Time × group interaction and post-hoc comparisons were verified using random effects models to analyse longitudinal evolution of variables.

    Article Snippet: The “sRAGE group” (n = 12) included animals with HCl-induced lung injury that also received intravenous treatment with sRAGE (3 mg.kg −1 ) (Recombinant Human RAGE Fc Chimera, R&D Systems, Minneapolis, MN).

    Techniques: Inhibition, Permeability, Whisker Assay, Recombinant

    RAGE inhibition decreases alveolar inflammation after acid-induced lung injury. Measurement of bronchoalveolar lavage (BAL) levels of ( A ) tumour necrosis factor (TNF)-α, ( B ) interleukin (IL)-6, ( C ) IL-1β and ( D ) IL-18 in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group). Values are reported as box and whisker plots. The Kruskal-Wallis test, with post-hoc Bonferroni test for pairwise comparisons were used.

    Journal: Scientific Reports

    Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets

    doi: 10.1038/s41598-019-45798-5

    Figure Lengend Snippet: RAGE inhibition decreases alveolar inflammation after acid-induced lung injury. Measurement of bronchoalveolar lavage (BAL) levels of ( A ) tumour necrosis factor (TNF)-α, ( B ) interleukin (IL)-6, ( C ) IL-1β and ( D ) IL-18 in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group). Values are reported as box and whisker plots. The Kruskal-Wallis test, with post-hoc Bonferroni test for pairwise comparisons were used.

    Article Snippet: The “sRAGE group” (n = 12) included animals with HCl-induced lung injury that also received intravenous treatment with sRAGE (3 mg.kg −1 ) (Recombinant Human RAGE Fc Chimera, R&D Systems, Minneapolis, MN).

    Techniques: Inhibition, Recombinant, Whisker Assay

    (A ) RAGE inhibition decreases histological features of lung injury. Lung injury scores were higher in acid-injured (HCl) than in uninjured piglets (Sham) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group). Values are reported as box and whisker plots. The Kruskal-Wallis test, with post-hoc Bonferroni test for pairwise comparisons were used. (B – E) Representative hematoxylin and eosin–stained sections at x20 original magnification of (B) uninjured piglets (Sham), (C) acid-injured piglets (HCl), acid-injured piglets treated with (D) RAGE antagonist peptide (HCl + RAP) or (E) recombinant sRAGE (HCl + sRAGE). There was greater cellularity consisting mainly of neutrophils (black arrowheads), more alveolar wall thickening (white arrowheads), and more areas of atelectasis and increased alveolar disruption, proteinous debris, and hemorrhage (black arrows) in untreated, acid-injured than in uninjured or treated piglets. Scale bars, 100 µm.

    Journal: Scientific Reports

    Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets

    doi: 10.1038/s41598-019-45798-5

    Figure Lengend Snippet: (A ) RAGE inhibition decreases histological features of lung injury. Lung injury scores were higher in acid-injured (HCl) than in uninjured piglets (Sham) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group). Values are reported as box and whisker plots. The Kruskal-Wallis test, with post-hoc Bonferroni test for pairwise comparisons were used. (B – E) Representative hematoxylin and eosin–stained sections at x20 original magnification of (B) uninjured piglets (Sham), (C) acid-injured piglets (HCl), acid-injured piglets treated with (D) RAGE antagonist peptide (HCl + RAP) or (E) recombinant sRAGE (HCl + sRAGE). There was greater cellularity consisting mainly of neutrophils (black arrowheads), more alveolar wall thickening (white arrowheads), and more areas of atelectasis and increased alveolar disruption, proteinous debris, and hemorrhage (black arrows) in untreated, acid-injured than in uninjured or treated piglets. Scale bars, 100 µm.

    Article Snippet: The “sRAGE group” (n = 12) included animals with HCl-induced lung injury that also received intravenous treatment with sRAGE (3 mg.kg −1 ) (Recombinant Human RAGE Fc Chimera, R&D Systems, Minneapolis, MN).

    Techniques: Inhibition, Recombinant, Whisker Assay, Staining, Disruption

    Measurement of net alveolar fluid clearance (AFC) rate as a marker of epithelial function in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl+RAP) or recombinant sRAGE (HCl+sRAGE) (n = 12 per group at each time point). Values are reported as medians and interquartile ranges.

    Journal: bioRxiv

    Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets

    doi: 10.1101/405423

    Figure Lengend Snippet: Measurement of net alveolar fluid clearance (AFC) rate as a marker of epithelial function in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl+RAP) or recombinant sRAGE (HCl+sRAGE) (n = 12 per group at each time point). Values are reported as medians and interquartile ranges.

    Article Snippet: The “sRAGE group” (n=12) included animals with HCl-induced lung injury that also received intravenous treatment with sRAGE (3 mg.kg -1 ) (RAGE (N-16) peptide, Santa Cruz Biotechnology, Dallas, TX, USA).

    Techniques: Marker, Recombinant

    Arterial oxygen tension (PaO 2 )/inspiratory oxygen fraction (FiO 2 ) in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl+RAP) or recombinant sRAGE (HCl+sRAGE) (n = 12 per group at each time point). Values are reported as medians and interquartile ranges.

    Journal: bioRxiv

    Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets

    doi: 10.1101/405423

    Figure Lengend Snippet: Arterial oxygen tension (PaO 2 )/inspiratory oxygen fraction (FiO 2 ) in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl+RAP) or recombinant sRAGE (HCl+sRAGE) (n = 12 per group at each time point). Values are reported as medians and interquartile ranges.

    Article Snippet: The “sRAGE group” (n=12) included animals with HCl-induced lung injury that also received intravenous treatment with sRAGE (3 mg.kg -1 ) (RAGE (N-16) peptide, Santa Cruz Biotechnology, Dallas, TX, USA).

    Techniques: Recombinant

    ( A ) Level of total protein in the bronchoalveolar lavage (BAL) fluid from uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl+RAP) or recombinant sRAGE (HCl+sRAGE) (n = 12 per group). ( B ) Extravascular lung water, as measured by transpulmonary thermodilution (Picco+, Pulsion SA) and indexed to body weight, in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl+RAP) or recombinant sRAGE (HCl+sRAGE) (n = 12 per group at each time point). Values are reported as medians and interquartile ranges.

    Journal: bioRxiv

    Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets

    doi: 10.1101/405423

    Figure Lengend Snippet: ( A ) Level of total protein in the bronchoalveolar lavage (BAL) fluid from uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl+RAP) or recombinant sRAGE (HCl+sRAGE) (n = 12 per group). ( B ) Extravascular lung water, as measured by transpulmonary thermodilution (Picco+, Pulsion SA) and indexed to body weight, in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl+RAP) or recombinant sRAGE (HCl+sRAGE) (n = 12 per group at each time point). Values are reported as medians and interquartile ranges.

    Article Snippet: The “sRAGE group” (n=12) included animals with HCl-induced lung injury that also received intravenous treatment with sRAGE (3 mg.kg -1 ) (RAGE (N-16) peptide, Santa Cruz Biotechnology, Dallas, TX, USA).

    Techniques: Recombinant

    Measurement of bronchoalveolar lavage (BAL) levels of ( A ) tumour necrosis factor (TNF)-α, ( B ) interleukin (IL)-6, ( C ) IL-1β and ( D ) IL-18 in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl+RAP) or recombinant sRAGE (HCl+sRAGE) (n = 12 per group). Values are reported as medians and interquartile ranges.

    Journal: bioRxiv

    Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets

    doi: 10.1101/405423

    Figure Lengend Snippet: Measurement of bronchoalveolar lavage (BAL) levels of ( A ) tumour necrosis factor (TNF)-α, ( B ) interleukin (IL)-6, ( C ) IL-1β and ( D ) IL-18 in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl+RAP) or recombinant sRAGE (HCl+sRAGE) (n = 12 per group). Values are reported as medians and interquartile ranges.

    Article Snippet: The “sRAGE group” (n=12) included animals with HCl-induced lung injury that also received intravenous treatment with sRAGE (3 mg.kg -1 ) (RAGE (N-16) peptide, Santa Cruz Biotechnology, Dallas, TX, USA).

    Techniques: Recombinant

    Lung injury scores were higher in acid-injured (HCl) than in uninjured piglets (Sham) and acid-injured piglets treated with RAGE antagonist peptide (HCl+RAP) or recombinant sRAGE (HCl+sRAGE) (n = 12 per group). Values are reported as medians and interquartile ranges.

    Journal: bioRxiv

    Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets

    doi: 10.1101/405423

    Figure Lengend Snippet: Lung injury scores were higher in acid-injured (HCl) than in uninjured piglets (Sham) and acid-injured piglets treated with RAGE antagonist peptide (HCl+RAP) or recombinant sRAGE (HCl+sRAGE) (n = 12 per group). Values are reported as medians and interquartile ranges.

    Article Snippet: The “sRAGE group” (n=12) included animals with HCl-induced lung injury that also received intravenous treatment with sRAGE (3 mg.kg -1 ) (RAGE (N-16) peptide, Santa Cruz Biotechnology, Dallas, TX, USA).

    Techniques: Recombinant

    The gene expression levels of α1-ENaC (NCBI Reference Sequence, NM_213758.2), α1-Na,K-ATPase (NM_214249.1), AQP-5 (NM_001110424.1) and 36B4 (a housekeeping gene) were assessed using the quantitative real-time reverse transcription polymerase chain reaction in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl+RAP) or recombinant sRAGE (HCl+sRAGE) (n = 12 per group). Threshold levels of mRNA expression (ΔΔCt) were normalised to the housekeeping gene 36B4 and the values represent the mean of triplicate samples ± SD. Data are representative of three independent observations.

    Journal: bioRxiv

    Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets

    doi: 10.1101/405423

    Figure Lengend Snippet: The gene expression levels of α1-ENaC (NCBI Reference Sequence, NM_213758.2), α1-Na,K-ATPase (NM_214249.1), AQP-5 (NM_001110424.1) and 36B4 (a housekeeping gene) were assessed using the quantitative real-time reverse transcription polymerase chain reaction in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl+RAP) or recombinant sRAGE (HCl+sRAGE) (n = 12 per group). Threshold levels of mRNA expression (ΔΔCt) were normalised to the housekeeping gene 36B4 and the values represent the mean of triplicate samples ± SD. Data are representative of three independent observations.

    Article Snippet: The “sRAGE group” (n=12) included animals with HCl-induced lung injury that also received intravenous treatment with sRAGE (3 mg.kg -1 ) (RAGE (N-16) peptide, Santa Cruz Biotechnology, Dallas, TX, USA).

    Techniques: Gene Expression, Sequencing, Reverse Transcription, Polymerase Chain Reaction, Recombinant, Expressing